1. Fate of the replisome following arrest by UV-induced DNA ...

    Fate of the replisome following arrest by UV-induced DNA damage in Escherichia coli H. Arthur Jeiranian, Brandy J. Schalow, Charmain T. Courcelle, and Justin Courcelle1 Department of Biology, Portland State University, Portland, OR 97201

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  2. Uptake and Fate of Fluorescently Labeled DNA ...

    Fluorescent dye labeling of DNA oligonucleotides and nanostructures is one of the most used techniques to track their fate and cellular localization inside cells. Here, we report that intracellular fluorescence, and even FRET signals, cannot be correlated with the cellular uptake of intact DNA structures. Live cell imaging revealed high colocalization of cyanine-labeled DNA oligos and ...

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  3. Synthetic transcription factors for cell fate ...

    Syn-TFs consist of a modular DNA-binding domain coupled with a catalytic or scaffold domain that can modulate gene expression when localized to genomic regulatory elements. ... When targeting an endogenous gene encoding a master transcription factor that mediates cell fate conversion, Syn-TFs become dispensable and expression of the target gene ...

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  4. Issue: Cell

    The proliferation-senescence cell-fate decision after drug treatment is determined by three distinct patterns of p21 dynamics. Either a delayed or acute drug-induced p21 response leads to a senescence fate, while an intermediate pulse of p21 response leads to a proliferation fate.

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  5. A Golden Gate Modular Cloning Toolbox for Plants | ACS ...

    A Bacterial Expression Vector Archive (BEVA) for Flexible Modular Assembly of Golden Gate-Compatible Vectors. Frontiers in Microbiology 2019, 9 DOI: 10.3389/fmicb.2018.03345. Baptiste Castel, Laurence Tomlinson, Federica Locci, Ying Yang, Jonathan D. G. Jones, . Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis.

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  6. Modular cis-regulatory organization of Endo16, a gut ...

    cis-regulatory system is modular in functional organization, in that subregions containing sites for specific sets of DNA-protein interaction carry out particular regulatory roles. Some of the modules function positively, mediating expression in vegetal plate …

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  7. The Gartner Lab

    Controlling the valency of targeting elements on nanoparticles is a major challenge. Here we describe a modular and scalable synthetic strategy to generate monovalent semiconductor quantum dots by steric exclusion. Phosphorothioate DNA is mixed with ZnS coated CdSe QDs to produce targetable and highly fluorescent nanoparticles of a single valency.

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  8. Christina Smolke's Profile | Stanford Profiles

    Christina Smolke is part of Stanford Profiles, official site for faculty, postdocs, students and staff information (Expertise, Bio, Research, Publications, and more). The site facilitates research and collaboration in academic endeavors.

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  9. Modular Organization Genes

    ModularOrganization ofGenesRequiredfor ComplexPolyketide Biosynthesis STEFANODONADIO,MICHAELJ. STAVER, JAMESB. MCALPINE, SUSANJ. SWANSON,LEONARDKATZ In Saccharopolyspora erythraea, the genes that govern synthesis of the polyketide portion of the macrolide antibiotic erythromycin are organized in six repeated …

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  10. Biomedical Polymer Science Laboratory

    By showing that the modular system based upon concentration of DNA at the level of the cell can be used to increase transfection efficiency, we have shown that further modification of the system may better target DNA delivery and overcome other barriers of DNA expression.

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  11. Building in vitro transcriptional regulatory networks by ...

    Aug 19, 2019· The regulation of cellular dynamics and responses to stimuli by genetic regulatory networks suggests how in vitro chemical reaction networks might analogously direct the dynamics of …

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  12. - YouTube

    Unsubscribe from Good Simple Living And Homesteading? Cancel Unsubscribe. Working...

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  13. CYCLOPS, A DNA-Binding Transcriptional Activator ...

    Singh et al. identify the Calcium- and Calmodulin-dependent Kinase (CCaMK) substrate CYCLOPS, as a phosphorylation-dependent and sequence-specific DNA-binding transcriptional activator essential for plant root symbiosis. A phosphomimetic CYCLOPS mutant triggers root nodules in the absence of CCaMK, pinpointing CYCLOPS as a master regulator of the symbiotic nodule organogenesis program.

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  14. Resource - limlab.ucsf.edu

    activate sets of genes associated with a new cell fate while simultaneously repressing sets of genes associated with a prior or alternative fate. Similarly, environmental conditions often ... inherently modular and programmable: DNA targets can be recognized by base pairing, and modular RNA-protein interac ...

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  15. Cellular Fate of a Modular DNA Delivery System Mediated by ...

    Each modular component can be designed to circumvent each of the many barriers. The modular approach will allow modification of individual components for a specific application. By utilizing a dense silica nanoparticle to form a ternary complex, transfection efficiency of a DNA‐transfection reagent complex was increased by a factor of ...

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  16. Transcription factor - Wikipedia

    In molecular biology, a transcription factor (TF) (or sequence-specific DNA-binding factor) is a protein that controls the rate of transcription of genetic information from DNA to messenger RNA, by binding to a specific DNA sequence. The function of TFs is to regulate—turn on and off—genes in order to make sure that they are expressed in the right cell at the right time and in the right ...

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  17. : Online Shopping for Electronics, Apparel ...

    Online shopping from the earth's biggest selection of books, magazines, music, DVDs, videos, electronics, computers, software, apparel & accessories, shoes, jewelry ...

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  18. 2 CB Flashcards | Quizlet

    Each chromosome is a single molecule of ____ whose extraordinarily, long length is most highly compacted during ____. This is accomplished by the DNA binding to _____ that package the DNA. _____ is/are the basic unit structure of eukaryotic chromatin. Genes that are being transcribed are packaged in a _____ condensed type of chromatin.

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  19. Transcriptional activation: risky business

    In this model, a DNA-bound activator (A), with its modular DNA-binding domain (DBD) and transcriptional activation domain (TAD), interacts with components of the basal transcriptional apparatus (shown in blue) and recruits these components to the promoter (B), signaling transcription

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  20. Student Publications | SSPB | Rice University

    Systems, Synthetic, and Physical Biology is a rapidly growing field that has the potential to transform every aspect of society. Rice University has been investing in these areas for over a decade by hiring faculty, establishing the NSF Center for Theoretical Biological Physics, and building core infrastructure that enables SSPB-type of research.

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  21. Developmental Biology Exam 1 Practice Questions and Study ...

    (a) One DNA molecule becomes two identical ones as a result of this process. (DNA is replicated in meiosis and mitosis, unzips along base pair chemical bonds. Free nucleotides connect to the exposed base pairs on both strands resulting in the formation of the missing side of each new DNA)

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  22. Designing modular reaction-diffusion programs for complex ...

    Designing modular reaction-diffusion programs for complex pattern formation Dominic Scalise1 & Rebecca Schulman1,2 Cells use sophisticated, multiscale spatial patterns of chemical instructions to control cell fate and tissue growth. While som e types

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  23. REVIEW Open Access Evolution of transcription factor ...

    Modular DNA binding B Tissue--specific alternative splicing Post-translational modification P C Figure 1 Mechanisms for generating transcription factor diversity and limiting novel function to specific contexts. Many of these mechanisms are modular and may be mixed and matched to offer even greater evolutionary flexibility. A.

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  24. Modular design of artificial transcription factors ...

    Dec 01, 2002· This has led to the design of a wide variety of modular artificial transcription factors (ATFs) that can stimulate or inhibit the expression of targeted genes. ... it is the choice of genes that are expressed which governs the fate and function of cells. ... The DNA-binding modules of transcriptional regulators consist of a wide variety of ...

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  25. Fate of modular cardiac tissue constructs in a syngeneic ...

    Journal of tissue engineering and regenerative medicine 2013-3-19 Fate of modular cardiac tissue constructs in a syngeneic rat model. [Brendan M Leung, Yasuo Miyagi, Ren-Ke Li, Michael V Sefton]

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  26. Reprogramming cell fate with a genome-scale library of ...

    Dec 20, 2016· The ability to convert cells into desired cell types enables tissue engineering, disease modeling, and regenerative medicine; however, methods to generate desired cell types remain difficult, uncertain, and laborious. We developed a strategy to screen gene regulatory elements on a genome scale to discover paths that trigger cell fate changes.

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  27. CRISPR-Mediated Modular RNA-Guided Regulation of ...

    Our results establish that the CRISPR system can be used as a modular and flexible DNA-binding platform for the recruitment of proteins to a target DNA sequence, revealing the potential of CRISPRi as a general tool for the precise regulation of gene expression in eukaryotic cells.

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  28. Asuka Eguchi's Profile | Stanford Profiles

    Asuka Eguchi is part of Stanford Profiles, official site for faculty, postdocs, students and staff information (Expertise, Bio, Research, Publications, and more). The site facilitates research and collaboration in academic endeavors.

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  29. Asuka Eguchi - Postdoctoral Fellow - Stanford University ...

    Because DNA-binding factors are modular in design, they can be engineered to target specific genomic sequences and perform pre-programmed regulatory functions upon binding.

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  30. Diversity among POU transcription factors in chromatin ...

    Diversity among POU transcription factors in chromatin recognition and cell fate reprogramming. ... protein family is an evolutionary ancient group of transcription factors (TFs) that bind specific DNA sequences to direct gene expression programs. ... Their modular architecture endows POU TFs with the capacity to accommodate alternative ...

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  31. A modular method for the extraction of DNA and RNA, and ...

    May 19, 2015· Higher DNA yields were achieved with this modular protocol than with several widely used commercial kits for the extraction of DNA and/or RNA from soil and filtrates. Given that the active ingredients of various commercial protocols are proprietary, it is not possible to identify why this modular protocol yielded higher DNA or RNA yields.

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  32. Reprogramming cell fate with a genome-scale library of ...

    tory networks that govern cell fate conversion. TFs are modular by nature, and each domain can be tailored to create ATFs that target and regulate genes and networks in a preprogrammed manner (11–14). The DNA-binding domain (DBD) confers sequence specificity in targeting genomic loci. The

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  33. Reprogramming cell fate with artificial transcription factors

    that condense genomic DNA to be inaccessible to transcription factors. Examples of activation and repression domains are provided in the box. 890 FEBS Letters 592 (2018) 888–900 ª 2018 Federation of European Biochemical Societies Reprogramming cell fate with ATFs E. A. Heiderscheit et al.

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